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19 The primers used for RT-PCR is listed below: GAPDH F: 5′-TGGCCTTCCGTGTTCCTAC-3′ GAPDH R: 5′-GAGTTGCTGTTGAAGTCGCA-3′ IL1A F: 5′-CGAAGACTACAGTTCTGCCATT-3′ IL1A R: 5′-GACGTTTCAGAGGTTCTCAGAG-3′ IL1B F: 5′-GCAACTGTTCCTGAACTCAACT-3′ IL1B R: 5′-ATCTTTTGGGGTCCGTCAACT-3′ IL6 F: 5′-TAGTCCTTCCTACCCCAATTTCC-3′ IL6 R: 5′-TTGGTCCTTAGCCACTCCTTC-3′ MMP3 F: 5′-ACATGGAGACTTTGTCCCTTTTG-3′ MMP3 R: 5′-TTGGCTGAGTGGTAGAGTCCC-3′ MMP9 F: 5′-CTGGACAGCCAGACACTAAAG-3′ MMP9 R: 5′-CTCGCGGCAAGTCTTCAGAG-3′ CCL2 F: 5′-TTAAAAACCTGGATCGGAACCAA-3′ CCL2 R: 5′-GCATTAGCTTCAGATTTACGGGT-3′ CCL4 F: 5′-TTCCTGCTGTTTCTCTTACACCT-3′ CCL4 R: 5′-CTGTCTGCCTCTTTTGGTCAG-3′ BCL2 F: 5′-GTCGCTACCGTCGTGACTTC-3′ BCL2 R: 5′-CAGACATGCACCTACCCAGC-3′ BCL2A1A F: 5′-GGCTGAGCACTACCTTCAGTA-3′ BCL2A1A R: 5′-TGGCGGTATCTATGGATTCCAC-3′ CCND2 F: 5′-GAGTGGGAACTGGTAGTGTTG-3′ CCND2 R: 5′-CGCACAGAGCGATGAAGGT-3′ RNA-Sequencing (RNA-Seq) library preparation and data analysis For RNA-Seq library preparation, total RNA was first extracted using Trizol, as previously described, 21, 22 from control and ESCC tissues ( n =3 each)